Missense3D-DB is a database resource, which contains pre-computed atom-based calculations of the impact of amino acid substitution on protein structure obtained using the Missense3D algorithm. The current version of the database contains ~ 4 million missense variants from the following resources: Humsavar, ClinVar and gnomAD. Currently Missense3D-DB hosts variants prediction based on what we consider the best representative 3D coordinates for the query protein. Additional 3D coordinates representing the query protein in different conformational states or in complex with ligands or other proteins may be available. If you want to make predictions using different 3D coordinates please visit our variant prediction Missense3D software. Missense3D-DB is freely available to academic and commercial users.